mouse antibody against stat3  (Cell Signaling Technology Inc)

Journal: Cell Death & Disease

Article Title: Super enhancer lncRNA RP11-54O7.17 regulates the proliferation and metastasis of triple-negative breast cancer by targeting lysosomal degradation of S100A4

doi: 10.1038/s41419-025-08072-3

Figure Lengend Snippet: A Detection of RP11-54O7.17 subcellular localization by in situ hybridization, n = 3. B Subcellular localization of RP11-54O7.17 by nucleocytoplasmic separation assay, n = 3. C Schematic representation of related genes downstream of the SE region where RP11-54O7.17 is located. D Changes in SE-related downstream genes after RP11-54O7.17 overexpression detected by RT-qPCR assay, n = 3. E Analysis of transcription factors related to RP11-54O7.17 expression. F Effect of RP11-54O7.17 overexpression on STAT3 cellular localization detected by immunofluorescence, n = 3. G Expression of p-STAT3 and STAT3 in RP11-54O7.17 overexpression TNBC cells detected by Western Blotting, n = 3. H Intranuclear and extranuclear STAT3 content in RP11-54O7.17 overexpressing cells detected by nucleocytoplasmic separation assay, n = 3. Data for ( B and D ) are presented as mean ± SD, * P < 0.05, ** P < 0.01.

Article Snippet: Primary antibodies in the study were antibodies against p-STAT3 (1:1000, Cst, #4113), STAT3 (1:1000, Cst, #30835), S100A4 (1:1000, Cst, #13018), HSP70 (1:1000, Santa Cruz, #K2020), GAPDH (1:1000, Cst, #5174), β-tubulin (1:1000, Cst, #2128) and H3 (1:1000, Millipore, #06-599).

Techniques: In Situ Hybridization, Over Expression, Quantitative RT-PCR, Expressing, Immunofluorescence, Western Blot

Journal: Cell Death & Disease

Article Title: Super enhancer lncRNA RP11-54O7.17 regulates the proliferation and metastasis of triple-negative breast cancer by targeting lysosomal degradation of S100A4

doi: 10.1038/s41419-025-08072-3

Figure Lengend Snippet: A Expression of S100A4 and p-STAT3 in RP11-54O7.17 overexpressed cells detected by Western blotting, n = 3. B Expression of S100A4 in RP11-54O7.17 overexpressed cells detected by immunofluorescence (White arrows: S100A4 nucleoli enrichment, DAPI micronuclei), n = 3. C Expression of S100A4 in cells transfected with different concentrations of RP11-54O7.17 in vitro, n = 3. D Effect of S100A4 overexpression on the inhibition of cell clone formation induced by RP11-54O7.17 by colony formation assay, n = 3. E Effect of S100A4 overexpression on the inhibition of cell viability induced by RP11-54O7.17 by MTT assay, n = 3. F Effect of S100A4 knocked-down on STAT3 activation, n = 3. G Effect of S100A4 overexpression on STAT3 activation, n = 3. Data for ( D , E ) are presented as mean ± SD, ** P < 0.01, *** P < 0.001.

Article Snippet: Primary antibodies in the study were antibodies against p-STAT3 (1:1000, Cst, #4113), STAT3 (1:1000, Cst, #30835), S100A4 (1:1000, Cst, #13018), HSP70 (1:1000, Santa Cruz, #K2020), GAPDH (1:1000, Cst, #5174), β-tubulin (1:1000, Cst, #2128) and H3 (1:1000, Millipore, #06-599).

Techniques: Expressing, Western Blot, Immunofluorescence, Transfection, In Vitro, Over Expression, Inhibition, Colony Assay, MTT Assay, Activation Assay

Journal: Cell Death & Disease

Article Title: Super enhancer lncRNA RP11-54O7.17 regulates the proliferation and metastasis of triple-negative breast cancer by targeting lysosomal degradation of S100A4

doi: 10.1038/s41419-025-08072-3

Figure Lengend Snippet: A RP11-54O7.17 Liposome delivery modality. B Changes in nude mice body weight after RP11-54O7.17 liposome injection, n = 6. C Images of subcutaneous tumors, n = 6. D Weight of subcutaneous tumors, n = 6. E Changes in subcutaneous tumor volume, n = 6. F Content of RP11-54O7.17 tumor tissue, n = 6. G Expression of S100A4, p-STAT3 and Ki-67 in tumor tissues detected by immunohistochemistry, n = 3. H Expression of p-STAT3 and S100A4 in tumor tissues detected by Western blotting, n = 3. I Expression of LC3B and P62 in tumor tissues detected by Western blotting, n = 3. Data for ( B , D , E , and F ) are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Primary antibodies in the study were antibodies against p-STAT3 (1:1000, Cst, #4113), STAT3 (1:1000, Cst, #30835), S100A4 (1:1000, Cst, #13018), HSP70 (1:1000, Santa Cruz, #K2020), GAPDH (1:1000, Cst, #5174), β-tubulin (1:1000, Cst, #2128) and H3 (1:1000, Millipore, #06-599).

Techniques: Injection, Expressing, Immunohistochemistry, Western Blot

Journal: Cell Death & Disease

Article Title: Super enhancer lncRNA RP11-54O7.17 regulates the proliferation and metastasis of triple-negative breast cancer by targeting lysosomal degradation of S100A4

doi: 10.1038/s41419-025-08072-3

Figure Lengend Snippet: RP11-54O7.17 is low-expressed and destabilized in TNBC cells, and the RP11-54O7.17 overexpression enhances the interaction of S100A4 with HSP70, promoting the autophagy-lysosome degradation of S100A4, which in turn suppresses the transcription of STAT3 downstream oncogenes.

Article Snippet: Primary antibodies in the study were antibodies against p-STAT3 (1:1000, Cst, #4113), STAT3 (1:1000, Cst, #30835), S100A4 (1:1000, Cst, #13018), HSP70 (1:1000, Santa Cruz, #K2020), GAPDH (1:1000, Cst, #5174), β-tubulin (1:1000, Cst, #2128) and H3 (1:1000, Millipore, #06-599).

Techniques: Over Expression

Journal: Physiological Reports

Article Title: Plasma hepcidin level is elevated by water immersion‐induced central fatigue via hepatic inflammatory response in male and female rats

doi: 10.14814/phy2.70468

Figure Lengend Snippet: Hepatic inflammatory signaling was enhanced in the central fatigue group. Representative immunoblots of pSTAT3 tyr705 , STAT3, and α‐tubulin in liver lysates in each fatigue group (Panel a) and their densitometries (Panels b and c) ( n = 6). Levels of IL‐6 in the liver lysate in each fatigue group were evaluated (panel d) ( n = 6). Significant differences between groups were evaluated by two‐way ANOVA followed by Tukey's honestly significant difference test. pSTAT3 tyr705 : Phosphorylated signal transducers and activator of transcription 3 at tyrosine 705. IL‐6, interleukin‐6; STAT3, signal transducers and activator of transcription 3.

Article Snippet: After blocking with TBS containing 0.005% Tween 20 and 5% milk, the membranes were incubated overnight with primary antibodies against total Stat3 (#9139, Cell Signaling, RRID:AB_331757, 1:1000 dilution), phospho‐Stat3 (Tyr705) (#9145, Cell Signaling, RRID:AB_2491009, 1:1000 dilution), and α‐Tubulin (#ab4074, Abcam, RRID:AB_2288001, 1:10000 dilution).

Techniques: Western Blot